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KMID : 0380620080400020207
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Korean Journal of Food Science and Technology
2008 Volume.40 No. 2 p.207 ~ p.214
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Physiological Activity/Nutrition : Effect of the Hexane Extract of Saussurea Lappa on the Growth of HT-29 Human Colon Cancer Cells
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Kim Eun-Ji
Park Hee-Sook
Lim Soon-Sung
Kim Jeong-Sang
Shin Hyun-Kyung
Yoon Jung-Han
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Abstract
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In Asia Saussurea lappa (SL) has been used as a traditional herbal medicine to treat abdominal pain and tenesmus. Recently, in vitro cell culture studies have shown that SL has anti-ulcer, anti-inflammatory, and anti-tumor properties. To explore its potential chemopreventive and chemotherapeutic effects in colon cancer, we examined whether the hexane extract of SL (HESL) could inhibit the growth of HT-29 human colon cancer cells, and investigated the mechanisms for this effect. The cells were cultured with various concentrations (0-5 μg/mL) of HESL. The results indicated that HESL markedly decreased the numbers of viable HT-29 cells; whereas at the concentration of 5 μg/mL, HESL slightly decreased the viable cell numbers of CCD 1108Sk human skin normal fibroblasts at 72 hr. HESL substantially increased the numbers of cells in the sub G1 phase, and dose-dependently increased apoptotic cell numbers. Western blot analysis of the total cell lysates revealed that HESL increased Bax protein levels, but did not affect Bcl-2 levels. HESL induced the cleavage of poly (ADP-ribose) polymerase and caspases 8, 9, 7, and 3. This study demonstrated that HESL inhibits cell growth and induces apoptosis in HT-29 cells, which may be mediated by its ability to increase Bax levels and activate the caspase pathway. These findings may lead to the development of new therapeutic strategies for colon cancer treatment.
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KEYWORD
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Saussurea Lappa, colon cancer, apoptosis, Bax, caspase
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